The text of the feature says "You have advantage on attack rolls against any creature that hasn’t taken a turn in the combat yet." It's really that simple. Once they've taken a turn, you no longer get Advantage due to being an Assassin. Even if they spend that first turn surprised, or otherwise Incapacitated, they're still taking a turn, so your Advantage doesn't last until they "do something". Granted, if they're still Incapacitated or something similar on your SECOND turn in combat, you're probably getting Advantage another way :)
Now, let's say you have a situation where the Initiative order goes 1) You, 2) Your Ally, then 3) The Bad Guy. If your ally has a way to grant you an attack as a reaction on their turn(Battle Masters and Purple Dragon Knights have ways to do this, for example), you would still have Advantage because the Bad Guy still hasn't had a turn.
The other possibility to consider is if a new enemy joins the battle mid-way through. If they roll Initiative and end up below you in the turn order, I *believe* you get a chance to attack them with Advantage. But it'll depend on how the DM has brought them in. If it's the third brother of the two Hill Giants you're fighting, they probably rolled Initiative before you noticed them and got a turn before you knew they were in the battle. If one of your enemies summoned/conjured them in, and the summoned creature rolls its own Initiative, it's possible you'll get to act before they do. In that case, I believe Assassinate should be in play.
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The Assassins ability to have advantage vs enemies that have not acted yet. Is this every turn? Or is it just the first round of combat?
The text of the feature says "You have advantage on attack rolls against any creature that hasn’t taken a turn in the combat yet." It's really that simple. Once they've taken a turn, you no longer get Advantage due to being an Assassin. Even if they spend that first turn surprised, or otherwise Incapacitated, they're still taking a turn, so your Advantage doesn't last until they "do something". Granted, if they're still Incapacitated or something similar on your SECOND turn in combat, you're probably getting Advantage another way :)
Now, let's say you have a situation where the Initiative order goes 1) You, 2) Your Ally, then 3) The Bad Guy. If your ally has a way to grant you an attack as a reaction on their turn(Battle Masters and Purple Dragon Knights have ways to do this, for example), you would still have Advantage because the Bad Guy still hasn't had a turn.
The other possibility to consider is if a new enemy joins the battle mid-way through. If they roll Initiative and end up below you in the turn order, I *believe* you get a chance to attack them with Advantage. But it'll depend on how the DM has brought them in. If it's the third brother of the two Hill Giants you're fighting, they probably rolled Initiative before you noticed them and got a turn before you knew they were in the battle. If one of your enemies summoned/conjured them in, and the summoned creature rolls its own Initiative, it's possible you'll get to act before they do. In that case, I believe Assassinate should be in play.